@article {1925,
	title = {Elizabethkingia miricola infection in Chinese spiny frog (Quasipaa spinosa)},
	journal = {Transboundary and Emerging Diseases},
	volume = {66},
	year = {2019},
	pages = {1049-1053},
	abstract = {<p>Abstract Elizabethkingia miricola is a Gram-negative rod which has been incriminated in severe infections in humans. Recently, a serious infectious disease was identified in Chinese spiny frogs (Quasipaa spinosa), in the Sichuan Province of China; the disease was characterized by corneal opacity, the presence of ascites and neurological symptoms. A Gram-negative bacillus was isolated from the liver, spleen and kidney of the diseased frogs. Experimental infection test revealed that the bacillus could infect the frogs Q.\&nbsp;spinosa and the LD50 value was 1.19\&nbsp;{\texttimes}\&nbsp;106\&nbsp;cfu per frog. The isolated Gram-negative bacillus was identified as E.\&nbsp;miricola according to phenotypic characteristics, 16S rRNA and gyrB gene sequence analysis. The isolated strain was only susceptible to florfenicol among all investigated chemotherapeutic agents. Histological examination revealed that E.\&nbsp;miricola infection caused pathological lesions to multiple organs and tissues, especially in the liver, brain, kidney. These results confirmed that E.\&nbsp;miricola is an emerging pathogen of Chinese spiny frogs.</p>
},
	keywords = {characterization, Elizabethkingia miricola, lesions, outbreak, pathological, Quasipaa spinosa},
	doi = {https://doi.org/10.1111/tbed.13101},
	url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13101},
	author = {Lei, Xue Ping and Yi, Geng and Wang, Kai Yu and OuYang, Ping and Chen, De Fang and Huang, Xiao Li and Huang, Chao and Lai, Wei Min and Zhong, Zi Jun and Huo, Chang Liang and Yang, Ze Xiao}
}
