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	title = {Elizabethkingia miricola infection in Chinese spiny frog (Quasipaa spinosa)},
	journal = {Transboundary and Emerging Diseases},
	volume = {66},
	year = {2019},
	pages = {1049-1053},
	abstract = {<p>Abstract Elizabethkingia miricola is a Gram-negative rod which has been incriminated in severe infections in humans. Recently, a serious infectious disease was identified in Chinese spiny frogs (Quasipaa spinosa), in the Sichuan Province of China; the disease was characterized by corneal opacity, the presence of ascites and neurological symptoms. A Gram-negative bacillus was isolated from the liver, spleen and kidney of the diseased frogs. Experimental infection test revealed that the bacillus could infect the frogs Q.\&nbsp;spinosa and the LD50 value was 1.19\&nbsp;{\texttimes}\&nbsp;106\&nbsp;cfu per frog. The isolated Gram-negative bacillus was identified as E.\&nbsp;miricola according to phenotypic characteristics, 16S rRNA and gyrB gene sequence analysis. The isolated strain was only susceptible to florfenicol among all investigated chemotherapeutic agents. Histological examination revealed that E.\&nbsp;miricola infection caused pathological lesions to multiple organs and tissues, especially in the liver, brain, kidney. These results confirmed that E.\&nbsp;miricola is an emerging pathogen of Chinese spiny frogs.</p>
},
	keywords = {characterization, Elizabethkingia miricola, lesions, outbreak, pathological, Quasipaa spinosa},
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