View source for Listeria monocytogenes (Infection): Dose Response Models

==<center>'''''Listeria monocytogenes''''' '''(Infection)'''</center>==

<center><big>'''Author: Sushil Tamrakar'''</big></center>


==='''General overview of ''Listeria monocytogenes'' '''===

''Listeria monocytogenes'' is a gram-positive rod-shaped bacterium. It is the causative agent of listeriosis, a serious infection caused by eating food contaminated with the bacteria. The disease generally affects older adults, pregnant women, newborns, and adults with weakened immune systems. However, rarely, persons without these risk factors can also be affected <ref name=CDC>[http://www.cdc.gov/listeria/ CDC]</ref> <ref name=Todar>[http://textbookofbacteriology.net/ Todar]</ref>

The overt form of the disease has mortality greater than 25 percent. The two main clinical manifestations are sepsis and meningitis. Meningitis is often complicated by encephalitis, a pathology that is unusual for bacterial infections <ref name=Todar></ref>. 




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==='''Summary Data'''===

Golnazarian et al.(1989)<ref name=Golnazarian></ref> also compared infectious dose in normal and compromised C57BL/6J mice with pathogens (strain F5817) via oral route and responses were recorded as death and infection separately. Audurier et al.(1980) <ref name=Audurier></ref> conducted an experiment on Swiss female mice (OF1) via oral route and infection was observed after 28 days of inoculation.The dose-response analysis of both cases were published by Haas, Thayyar-Madabusi in 1999 <ref name=Audurier></ref> <ref name=Thayyar-madabusi>Haas CN, Thayyar-Madabusi A, et al. (1999) Development and Validation of Dose-Response Relationship for Listeria monocytogenes. Quantitative Microbiology 1(1): 89-102.</ref>

{{DRSummaryTableStart|agent=''Listeria monocytogenes''}}
{{DRSummaryTablePreferredModel|expID= 292,295 Pooled |refer=Golnazarian|reference=Golnazarian, C. A., C. W. Donnelly, et al. (1989). "Comparison of infectious dose of Listeria monocytogenes F5817 as determined for normal versus compromised C57B1/6J mice." Journal of food protection 52(10): 696-701.|host= C57Bl/6J mice  |agentStrain= F5817 |route= oral  |nDoses= 10 |doseUnits= CFU |response= infection |bestFitModel=beta-Poisson|parameters=&#945; =  2.53E-01 , N<sub>50</sub> =  2.77E+02 |N50= 2.77E+02 }}
{{DRSummaryTableNonpreferredModel|expID= 292 |refer=Golnazarian|reference=Golnazarian, C. A., C. W. Donnelly, et al. (1989). "Comparison of infectious dose of Listeria monocytogenes F5817 as determined for normal versus compromised C57B1/6J mice." Journal of food protection 52(10): 696-701.|host= C57Bl/6J mice |agentStrain=F5817|route= oral  |nDoses= 6 |doseUnits= CFU |response= infection |bestFitModel=beta-Poisson|parameters=&#945; =  6.95E-01 , N<sub>50</sub> =  3.39E+03 |N50= 3.39E+03 }}
{{DRSummaryTableNonpreferredModel|expID=295  |refer=Golnazarian|reference=Golnazarian, C. A., C. W. Donnelly, et al. (1989). "Comparison of infectious dose of Listeria monocytogenes F5817 as determined for normal versus compromised C57B1/6J mice." Journal of food protection 52(10): 696-701.|host= C57Bl/6J mice|agentStrain= F5817 |route= oral  |nDoses= 4 |doseUnits= CFU |response= infection |bestFitModel=beta-Poisson|parameters=&#945; =  1.2E-01 , N<sub>50</sub> =  1.16E+03 |N50= 1.16E+03 }}
{{DRSummaryTableNonpreferredModel|expID= 296 |refer=Audurier|reference=Audurier, A., P. Pardon, et al. (1980). "Experimental infection of mice with Listeria monocytogenes and L. innocua." Annales de microbiologie 131B(1): 47-57.|host= OF1 mice |agentStrain= strain 10401 |route= oral  |nDoses= 5 |doseUnits= CFU |response= infection |bestFitModel=beta-Poisson|parameters=&#945; =  1.72E-01 , N<sub>50</sub> =  2.06E+06 |N50= 2.06E+06 }}
{{DRSummaryTableEnd}}


[[File:Exponential and betapoisson model.jpg|thumb|none|550px]]

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==='''Optimization Output for experiment 292 and 295 (Pooling)'''===


{{DRExperimentDataTable10|title=Pooled C57Bl/6J mice/''Listeria monocytogenes''|refer=Golnazarian|reference=Golnazarian, C. A., C. W. Donnelly, et al. (1989). "Comparison of infectious dose of Listeria monocytogenes F5817 as determined for normal versus compromised C57B1/6J mice." Journal of food protection 52(10): 696-701|pos=Infected|neg=Non-infected|d1=2|p1=0|n1=6|t1=6|d2=5|p2=1|n2=5|t2=6|d3=110|p3=2|n3=4|t3=6|d4=5500|p4=7|n4=3|t4=10|d5=32400|p5=7|n5=3|t5=10|d6=39000|p6=4|n6=2|t6=6|d7=55000|p7=9|n7=1|t7=10|d8=251000|p8=10|n8=0|t8=10|d9=550000|p9=10|n9=0|t9=10|d10=2820000|p10=10|n10=0|t10=10}}


{{DRFit|title=Goodness of fit and model selection|devE=35.7|devB=8.75|delta=26.9|DFE=9|DFB=8|X2bPbetter=3.84|pbPbetter=2.12e-07|X2GOFe=16.9|pGOFe=4.52e-05|X2GOFb=15.5|pGOFb=0.364|interpretation=Beta-Poisson fits better than exponential; cannot reject good fit for beta-Poisson.}}


{{DRConfidenceBetaPoisson|title=Optimized parameters for the beta-Poisson model, from 10000 bootstrap iterations|MLEa=2.53E-01|p5a=1.51E-01|p25a=1.62E-01|p50a=1.78E-01|p950a=6.47E-01|p975a=8.62E-01|p995a=1.79E+00|MLEN=2.77E+02|p5N=3.39E+01|p25N=5.48E+01|p50N=6.67E+01|p950N=4.48E+03|p975N=7.53E+03|p995N=1.29E+04}}


[[File:BPscatter ID292 295.png|thumb|left|500px|'''Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.''']][[File:BPmodel ID292 295.png|thumb|none|500px|'''beta Poisson model plot, with confidence bounds around optimized model''']]<br>


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==='''Optimization Output for experiment 292'''===


{{DRExperimentDataTable6|title=C57Bl/6J mice/''Listeria monocytogenes''|refer=Golnazarian|reference=Golnazarian, C. A., C. W. Donnelly, et al. (1989). "Comparison of infectious dose of Listeria monocytogenes F5817 as determined for normal versus compromised C57B1/6J mice." Journal of food protection 52(10): 696-701|pos=Infected|neg=Non-infected|d1=5500|p1=7|n1=3|t1=10|d2=32400|p2=7|n2=3|t2=10|d3=55000|p3=9|n3=1|t3=10|d4=251000|p4=10|n4=0|t4=10|d5=550000|p5=10|n5=0|t5=10|d6=2820000|p6=10|n6=0|t6=10}}


{{DRFit|title=Goodness of fit and model selection|devE=9.88|devB=3.4|delta=6.48|DFE=5|DFB=4|X2bPbetter=3.84|pbPbetter=0.0109|X2GOFe=11.1|pGOFe=0.0788|X2GOFb=9.49|pGOFb=0.494|interpretation=Beta-Poisson fits better than exponential; cannot reject good fit for beta-Poisson.}}


{{DRConfidenceBetaPoisson|title=Optimized parameters for the beta-Poisson model, from 10000 bootstrap iterations|MLEa=6.95E-01|p5a=2.69E-01|p25a=3.39E-01|p50a=3.78E-01|p950a=2.56E+00|p975a=2.28E+01|p995a=1.18E+03|MLEN=3.39E+03|p5N=3.58E+01|p25N=2.47E+02|p50N=4.67E+02|p950N=1.09E+04|p975N=1.26E+04|p995N=1.85E+04}}


[[File:BPscatter ID292.png|thumb|left|500px|'''Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.''']][[File:BPmodel ID292.png|thumb|none|500px|'''beta Poisson model plot, with confidence bounds around optimized model''']]<br>


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==='''Optimization Output for experiment 295'''===


{{DRExperimentDataTable4|title=C57Bl/6J mice/''Listeria monocytogenes''|refer=Golnazarian|reference=Golnazarian, C. A., C. W. Donnelly, et al. (1989). "Comparison of infectious dose of Listeria monocytogenes F5817 as determined for normal versus compromised C57B1/6J mice." Journal of food protection 52(10): 696-701|pos=Infected|neg=Non-infected|d1=2|p1=0|n1=6|t1=6|d2=5|p2=1|n2=5|t2=6|d3=110|p3=2|n3=4|t3=6|d4=39000|p4=4|n4=2|t4=6}}


{{DRFit|title=Goodness of fit and model selection|devE=25.8|devB=0.897|delta=24.9|DFE=3|DFB=2|X2bPbetter=3.84|pbPbetter=6.09e-07|X2GOFe=7.81|pGOFe=1.06e-05|X2GOFb=5.99|pGOFb=0.639|interpretation=Beta-Poisson fits better than exponential; cannot reject good fit for beta-Poisson.}}


{{DRConfidenceBetaPoisson|title=Optimized parameters for the beta-Poisson model, from 10000 bootstrap iterations|MLEa=1.2E-01|p5a=1.86E-02|p25a=3.62E-02|p50a=4.68E-02|p950a=1.85E+00|p975a=1.84E+01|p995a=1.29E+02|MLEN=1.16E+03|p5N=2.63E+01|p25N=6.05E+01|p50N=8.54E+01|p950N=2.68E+06|p975N=5.35E+07|p995N=1.51E+14}}


[[File:BPscatter ID295.png|thumb|left|500px|'''Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.''']][[File:BPmodel ID295.png|thumb|none|500px|'''beta Poisson model plot, with confidence bounds around optimized model''']]<br>


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==='''Optimization Output for experiment 296'''===

{{DRExperimentDataTable5|title=OF1 mice/''Listeria monocytogenes''|refer=Audurier|reference=Audurier, A., P. Pardon, et al. (1980). "Experimental infection of mice with Listeria monocytogenes and L. innocua." Annales de microbiologie 131B(1): 47-57.|pos=Infected|neg=Non-infected|d1=970000|p1=2|n1=3|t1=5|d2=9.7E+06|p2=3|n2=2|t2=5|d3=9.7E+07|p3=4|n3=1|t3=5|d4=9.7E+08|p4=5|n4=0|t4=5|d5=9.7E+09|p5=8|n5=2|t5=10}}


{{DRFit|title=Goodness of fit and model selection|devE=86.8|devB=2.6|delta=84.2|DFE=4|DFB=3|X2bPbetter=3.84|pbPbetter=0|X2GOFe=9.49|pGOFe=0|X2GOFb=7.81|pGOFb=0.458|interpretation=Beta-Poisson fits better than exponential; cannot reject good fit for beta-Poisson.}}


{{DRConfidenceBetaPoisson|title=Optimized parameters for the beta-Poisson model, from 10000 bootstrap iterations|MLEa=1.72E-01|p5a=1.81E-02|p25a=2.45E-02|p50a=3.24E-02|p950a=6.06E-01|p975a=9.07E-01|p995a=6.14E+00|MLEN=2.06E+06|p5N=1.03E-10|p25N=7.25E-06|p50N=8.26E-03|p950N=2.04E+07|p975N=3.39E+07|p995N=7.13E+07}}


[[File:BPscatter ID296.png|thumb|left|500px|'''Parameter scatter plot for beta Poisson model ellipses signify the 0.9, 0.95 and 0.99 confidence of the parameters.''']][[File:BPmodel ID296.png|thumb|none|500px|'''beta Poisson model plot, with confidence bounds around optimized model''']]<br>


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==='''Summary'''===

As the two data set of  C57 Bl/6 J  mice inoculated with ''L. monocytogenes'' <ref name=Golnazarian></ref> could be pooled. The pooled model is recommended to use as recommended model. However, according to Haas, Thayyar-Madabusi et al.(1999), the dose-response model of the experiment number 296 <ref name=Audurier></ref> showed consistence result to some reported outbreaks.

==='''References'''===

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[[Category:Completed Dose Response Models: Bacteria]][[Category:Dose Response Model]][[Category:Pseudomonas aeruginosa]]