Burkholderia pseudomallei
Author: Mark H. Weir
Overview
The bacterium Bukholderia pseudomallei (B. pseudomallei) is a gram negative bacterium and is present in numerous tropical regions such as Central and South America and Southeast Asia causes Melioidosis, typically infects horses, mules and donkeys but can also present a potentially life threatening disease in humans as well. Burkholderia mallei (B. mallei) is a close relative of B. pseudomallei both of which have been categorized as a B level bioterror agent by the CDC. No dose response models are currently available for B. mallei.
http://www.cdc.gov/melioidosis/
Liu et al. in 2002 examined infection through intranasal route to mimic infection through inhalation. C57BL/6 mice and BALB/c mice were inoculated intranasally to B. pseudomallei KHW strain and mortality was recorded as response (Liu, Koo et al. 2002). Miller et al.(1948) explored infection in guinea pigs via intraperitoneal route(Miller, Pannell et al. 1948). Similarly, Brett and Woods(1996) experimented infection in diabetic rats with B. pseuomallei 316c strain (Brett and Woods 1996.
Experiment serial number |
Reference |
Host type |
Agent strain |
Route |
# of doses |
Dose units |
Response |
Best fit model |
Optimized parameter(s) |
LD50/ID50
|
18,23* |
[1] |
C57BL/6 mice and diabetic rat |
KHW,316c |
intranasal,intraperitoneal |
10 |
CFU |
death |
beta-Poisson |
α = 3.28E-01 , N50 = 5.43E+03 |
5.43E+03
|
21,23 |
[2] |
guinea pig and diabetic rat |
W294, 316c |
intraperitoneal |
11 |
CFU |
death |
beta-Poisson |
α = 2.13E-01 , N50 = 4.77E+02 |
4.77E+02
|
18 |
[3] |
C57BL/6 mice |
KHW |
intranasal |
5 |
CFU |
infection |
exponential |
k = 1.00E-04 |
6.92E+03
|
17 |
[3] |
BALB/c mice |
KHW |
intranasal |
5 |
CFU |
infection |
exponential |
k = 1.04E-02 |
6.63E+01
|
21 |
[4] |
guinea pig |
W294 |
intraperitoneal |
6 |
CFU |
death |
beta-Poisson |
α = 2.67E-01 , N50 = 2.55E+02 |
2.55E+02
|
23 |
[5] |
diabetic rat |
316c |
intraperitoneal |
5 |
CFU |
death |
beta-Poisson |
α = 2.65E-01 , N50 = 2.27E+03 |
2.27E+03
|
*This model is preferred in most circumstances. However, consider all available models to decide which one is most appropriate for your analysis.
|
|
Recommended Model
Since the data of C57BL/6 mice and diabetic rat could be pooled and intranasal exposure is closer to inhalation which is the likeliest exposure route for humans, the pooled data sets and resulting models is preferred.
References
- ↑ Brett PJ and Woods DE (1996) Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates. Infection and Immunity 64(7), 2824-2828 and Liu B, Koo GC, Yap EH, Chua KL and Gan Y-H (2002) Model of Differential Susceptibility to Mucosal Burkholderia pseudomallei Infection. Infection and Immunity 70(2), 504-511.
- ↑ Miller WR, Pannell L, Cravitz L, Tanner WA and Rosebury T (1948) Studies on Certain Biological Characteristics of Malleomyces mallei and Malleomyces pseudomallei: II. Virulence and Infectivity for Animals. Journal of Bacteriology 55(1), 127-135. and Brett PJ and Woods DE (1996) Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates. Infection and Immunity 64(7), 2824-2828
- ↑ 3.0 3.1 3.2 3.3 Liu B, Koo GC, Yap EH, Chua KL and Gan Y-H (2002) Model of Differential Susceptibility to Mucosal Burkholderia pseudomallei Infection. Infection and Immunity 70(2), 504-511. Cite error: Invalid
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- ↑ 4.0 4.1 Miller WR, Pannel I, Cravitz I, Tanner WA and Rosebury T (1947) Studies on Certain Biological Characteristics of Melleomyces mallei and Malleomyces pseudomallei II. Virulence and Infectivity for Animals Journal of Bacteriology 55: 127-135 Cite error: Invalid
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- ↑ Brett PJ and Woods DE (1996) Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates. Infection and Immunity 64(7), 2824-2828
- ↑ 6.0 6.1 6.2 Liu B, Koo GC, Yap EH, Chua KL and Gan Y-H (2002) Model of Differential Susceptibility to Mucosal Burkholderia pseudomallei Infection. Infection and Immunity 70(2), 504-511. and Brett PJ and Woods DE (1996) Structural and immunological characterization of Burkholderia pseudomallei O-polysaccharide-flagellin protein conjugates. Infection and Immunity 64(7), 2824-2828. Cite error: Invalid
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