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General Overview

Pseudomonas aeruginosa causes bacteremia primarily in immunocompromised and immunosupressed patients. Hematologic malignancies, immunodeficiency relating to AIDS, diabetes mellitus, and severe burns are some of the preexisting conditions (Todar 2012). Any route including intravascular, intraperitoneal or intranasal routes of bacterial administration can cause ocular infection. An exposure to almost any bacterium able to cause severe bacteremia can result in ocular infection (Hazlett, Rosen et al. 1978).

http://www.cdc.gov/mmwr/preview/mmwrhtml/00001546.htm

Summary Data

Hazlett et al (1978) studied the susceptibility of newborn and infant mice to eye infection by P. aeruginosa. Inoculation of P. aeruginoa under the unopened eyelids of 5 and 10 day old mice resulted in acute infection and death of many animals due to severe bacteremia. [1]

Recommended Model

The pooled model of experiment number 281 and 282 was recommended model for bacteremia due to P. aeruginosa infection via eyes. The LD50 of the pooled model was lower than pooled model of experiment 283 and 284. Pooled model shows improvement in fitting than individual fits.

Exponential and betapoisson model.jpg

ID # of Doses Agent Strain Dose Units Host type Μodel Optimized parameters Response type Reference
297 & 298 10 CFU white rabbit beta-Poisson a = 1.9E-01

LD50/ID50 = 1.85E+04
N50 = 1.85E+04
corneal ulceration
297 5 CFU white rabbit beta-Poisson a = 3.55E-01

LD50/ID50 = 6.57E+03
N50 = 6.57E+03
corneal ulceration
297 5 CFU white rabbit beta-Poisson a = 3.55E-01

LD50/ID50 = 6.57E+03
N50 = 6.57E+03
corneal ulceration Effect of Pseudomonas aeruginosa concentration in experimental contact lens-related microbial keratitis
298 5 CFU white rabbit beta-Poisson a = 1.09E-01

LD50/ID50 = 1.52E+05
N50 = 1.52E+05
Severe stromal ulceration
286 5 ATCC 19660 CFU Swiss webster mice exponential
k = 8.8E-08
LD50/ID50 = 7.88E+06

infection (Keratitis) Age-Related Susceptibility to Pseudomonas aeruginosa Ocular Infections in Mice
281,282 (pooled) 12 ATCC 19660 CFU Swiss webster mice (5day old) exponential
k = 1.05E-04
LD50/ID50 = 6.61E+03

death
281 6 ATCC 19660 CFU Swiss webster mice (5day old) exponential
k = 8.52E-05
LD50/ID50 = 8.13E+03

death Age-Related Susceptibility to Pseudomonas aeruginosa Ocular Infections in Mice
Best Fit
Experiment ID:
297 & 298
# of Doses:
10
Agent Strain:
Dose Units:
CFU
Host type:
white rabbit
Μodel:
beta-Poisson
Optimized parameters: a = 1.9E-01

LD50/ID50 = 1.85E+04
N50 = 1.85E+04
Reference:
Experiment ID:
297
# of Doses:
5
Agent Strain:
Dose Units:
CFU
Host type:
white rabbit
Μodel:
beta-Poisson
Optimized parameters: a = 3.55E-01

LD50/ID50 = 6.57E+03
N50 = 6.57E+03
Reference:
Experiment ID:
297
# of Doses:
5
Agent Strain:
Dose Units:
CFU
Host type:
white rabbit
Μodel:
beta-Poisson
Optimized parameters: a = 3.55E-01

LD50/ID50 = 6.57E+03
N50 = 6.57E+03
Reference:
Experiment ID:
298
# of Doses:
5
Agent Strain:
Dose Units:
CFU
Host type:
white rabbit
Μodel:
beta-Poisson
Optimized parameters: a = 1.09E-01

LD50/ID50 = 1.52E+05
N50 = 1.52E+05
Reference:
Experiment ID:
286
# of Doses:
5
Agent Strain:
ATCC 19660
Dose Units:
CFU
Host type:
Swiss webster mice
Μodel:
exponential
Optimized parameters:
k = 8.8E-08
LD50/ID50 = 7.88E+06

Reference:
Best Fit
Experiment ID:
281,282 (pooled)
# of Doses:
12
Agent Strain:
ATCC 19660
Dose Units:
CFU
Host type:
Swiss webster mice (5day old)
Μodel:
exponential
Optimized parameters:
k = 1.05E-04
LD50/ID50 = 6.61E+03

Reference:
Experiment ID:
281
# of Doses:
6
Agent Strain:
ATCC 19660
Dose Units:
CFU
Host type:
Swiss webster mice (5day old)
Μodel:
exponential
Optimized parameters:
k = 8.52E-05
LD50/ID50 = 8.13E+03

Reference: